P19 Cell Line from mouse

95102107

• UNSPSC 코드: 41106514

속성

• 생물학적 소스: mouse embryo
• 설명: Mouse teratocarcinoma
• 성장 모드: Adherent
• 핵형: Euploid
• 형태학: Not specified
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Mouse teratocarcinoma

세포주 설명

The P19 cell line was derived from an embryonal carcinoma induced in a C3H/He strain mouse. The cells can be cloned at high efficiency in medium containing 0.1mM β-mercaptoethanol. The pluripotent P19 cells can be induced to differentiate into neuronal and glial cells in the presence of retinoic acid. Aggregates of P19 cells differentiate into cardiac and skeletal muscle in the presence of dimethyl sulfoxide (DMSO). In the presence of both retinoic acid and DMSO cells develop as if exposed to retinoic acid only.

애플리케이션

Differentiation studies

배양 배지

α MEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 2.5% Foetal Bovine Serum (FBS) + 7.5% Calf Serum (CS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

기타 정보

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