RIN-5F

95090402, rat pancreas, Not specified

• UNSPSC 코드: 41106514

속성

• product name: RIN-5F, 95090402
• 생물학적 소스: rat pancreas
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Not specified
• 제품: Insulin, L-dopa-decarboxylase
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Rat islet cell tumour

세포주 설명

RIN-5F is a secondary clone of the rat islet tumour cell line RIN-m (Sigma Catalogue number. 95071701). Cloning was performed by limited dilution in RPMI 1640, 10%FBS and 20% conditioned medium. The conditioned medium contained growth hormones and prolactin being produced by rat pituitary tumour cells GH3 (Sigma Catalogue number. 87012603). RIN-5F cells secrete insulin, produce high levels of the key APUD enzyme, L-dopa-decarboxylase and contain membrane-bound secretory granules.

애플리케이션

Study of pancreatic islet cell biology eg. synthesis, storage and secretion of insulin

배양 배지

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Cells grow in small islands and do not form a confluent monolayer.

기타 정보

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