PG

95070629, fish ovary, Fibroblast-like

• UNSPSC 코드: 41106514

속성

• product name: PG, 95070629
• 생물학적 소스: fish ovary
• 성장 모드: Adherent
• 핵형: Aneuploid
• 형태학: Fibroblast-like
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Fish Northern Pike ovaries

세포주 설명

The fish cell line PG has been initiated 1976 from ovarian tissue of 5 juvenile pike (Esox lucius). Cells grow at 4°C to 25°C with an optimum temperature of 20°C. PG cells are susceptible to various fish viruses including infectious pancreatic necosis viruses and pike fry rhabdovirus. The cell line has been mycoplasma eradicated at ECACC.

애플리케이션

Fish Virology

배양 배지

MEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 20°C Fish cell lines detach easily during transit if the culture is too young. After resuscitation split cells 1:3 to 1:4 and culture for at least one week wit

기타 정보

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