추천 제품
product name
DMS 79, 95062824
생물학적 소스
human lung
성장 모드
Aggregates in suspension
핵형
Aneuploid
형태학
Irregular
제품
Not specified
수용체
Not specified
기술
cell culture | mammalian: suitable
관련 질환(들)
cancer
배송 상태
dry ice
저장 온도
−196°C
세포주 기원
Human lung small cell carcinoma
세포주 설명
DMS79 has been established from pleural fluid of a patient with small cell carcinoma of the lung. The patient was treated with cytoxan, vincristine, methotrexate and radiation therapy. The cultures show a tendency for the modal chromosome number to decline. The production of adrenocorticotropin (ACTH), bombesin, calcitonin, β-endorphin, 17-β-estradiol, lipotropin, ocytocin-neurophysin, parathyroid hormone and somatstatin-like imuno-reactivity has been reported. The cells have also been reported to have the following cell surface antigens: Leu-7, Class I HLA, Class II HLA, Mg23 defined by the AML-2-23 monoclonal antibody and the AML-1-99 defined antigen. The cells have a receptor for epidermal growth factor (EGF) and produce tumours in nude athymic mice. Cells grow as irregularly shaped aggregates.
애플리케이션
Human lung tumour model, carcinogenesis studies.
배양 배지
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).
계대배양 정규 작업
Maintain cultures between 3 ? 9 x 100,000 cells/ml; 5% CO2; 37°C. Only dilute 1:2 as a maximum. This cell line grows as clumps in suspension which can be easily dispersed with pipetting. Trypsinisation is not necessary. Routine splitting can therefore
기타 정보
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