MES-SA

95051030, human uterus, Epithelial

• UNSPSC 코드: 41106514
• 생물학적 소스: human uterus
• 성장 모드: Adherent
• 핵형: Modal no. 45, monosomic forms of chromosomes 5, 6 and 7
• 형태학: Epithelial
• 제품: Not specified
• 수용체: Not specified
• 관련 질환(들): cancer

속성

• 제품명: MES-SA, 95051030
• 생물학적 소스: human uterus
• 성장 모드: Adherent
• 핵형: Modal no. 45, monosomic forms of chromosomes 5, 6 and 7
• 형태학: Epithelial
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human uterus sarcoma

세포주 설명

A human uterine sarcoma derived in 1980 from a tumour obtained at the time of a hysterectomy from a 56 year old Caucasian female. The cells form tumours in athymic nude mice, readily form colonies in soft agar and have a reported doubling time of 22-24 hours. The cells are sensitive to a number of chemotherapeutic agents including doxorubicin, dactinomycin, mitomycin C, taxol and bleomycin. They are resistant to vinblastine dacarbazine, cisplatin, melphalan, vincristine methotrexate and etoposide. The multi drug-resistant cell line MES-SA/Dx5 was established from MES-SA cells grown in the presence of doxorubicin.

애플리케이션

Tumourigenicity and chemotherapeutic agent studies

DNA 프로파일

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11,13
D16S539: 11,12
D5S818: 13
D7S820: 7,11
THO1: 6
TPOX: 8,11
vWA: 18

배양 배지

McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:6 to 1:8 i.e. seeding at 1-4 x 10,000 cells/cm² using 0.03% EDTA; 5% CO₂; 37°C. Do not use trypsin.

기타 정보

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