AFRC 17D1

94120825, Application: Fusion partner for hybridoma production

• UNSPSC 코드: 41106514

속성

• 생물학적 소스: Horse x Mouse/Horse Hybridoma
• ImmunoDon: Horse peripheral lymphocytes
• 골수종: NS0
• 반응성: Application: Fusion partner for hybridoma production
• 기술: cell culture | mammalian: suitable
• 동형: Not specified
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Horse peripheral blood lymphocytes x Mouse NS0

세포주 설명

The heterohybridoma AFRC 17D1 was obtained by fusion of lymphocytes from ponies immunised with the influenza A equine 2 virus (isolateA/Equine/Newmarket/79 (H3N8)) with mouse myeloma NS0 cells. The hybridoma was made aminopterin-sensitive by selective growth in 8-azaguanine. This non-Ig-secreting cell line was used as fusion partner for the establishment of equine-mouse heterohybidomas.

면역원

Influenza A equine 2 virus

애플리케이션

Fusion partner for hybridoma production

배양 배지

RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 5% Foetal Bovine Serum (FBS)

계대배양 정규 작업

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C

기타 정보

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