HF1-5

94101909, rat liver, Epithelial

• UNSPSC 코드: 41106514

속성

• product name: HF1-5, 94101909
• 생물학적 소스: rat liver
• 성장 모드: Adherent
• 핵형: 100 chromosomes
• 형태학: Epithelial
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Rat hepatoma Hybrid [H5xFao]

세포주 설명

HF1-5 is a subclone of HF1 selected for it′s ability to grow in glucose free medium. The entire group of hepatic functions are re-expressed. NB. The cells are difficult to cultivate, they do not like either low or high density and should be transferred frequently even though they grow slowly.

애플리케이션

Gene analysis and liver function expression.

배양 배지

Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split semi-confluent (60%-70%) cultures 1:5 i.e. seeding at 3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 37°C. Saturation density is reached at 5x1000,000 cells/10cm dish.

기타 정보

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