C2-Rev 7

94101907, rat liver, Epithelial

• UNSPSC 코드: 41106514

속성

• 제품명: C2-Rev 7, 94101907
• 생물학적 소스: rat liver
• 성장 모드: Adherent
• 핵형: Hyperdiploid, 51 chromosomes
• 형태학: Epithelial
• 제품: Albumin and hepatocyte markers
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Rat hepatoma [HGPRT-]

세포주 설명

C2-Rev 7 is a revertant of the C2 variants, selected by virtue of its capacity to grow in glucose free medium (containing dialysed serum) where survival requires activity of the liver-specific gluconeogenic pathway. Expression of the entire set of liver specific functions is restored. Albumin and hepatocyte markers present.

애플리케이션

Gene expression studies.

배양 배지

Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split semi-confluent cultures (70%) 1:10 i.e. seeding at 9x1000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Saturation density is reached at 15x1,000,000 cells/10cm² dish.

기타 정보

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.