BW1J

94101901, mouse skin, Epithelial

• UNSPSC 코드: 41106514

속성

• 제품명: BW1J, 94101901
• 생물학적 소스: mouse skin
• 포장: tube of 5 μg 94101901-DNA-5UG; pkg of vial of cells 94101901-1VL
• 성장 모드: Adherent
• 핵형: Hypotetraploid
• 형태학: Epithelial
• 제품: Alpha-foetoprotein and Albumin
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Mouse hepatoma

세포주 설명

A mouse hepatoma isolated from the BW7756 transplantable hepatoma carried in C57 leaden/J mice (see reference: Differentiation). Will synthesise in appreciable amounts only hepatic proteins that appear early in foetal development. The majority of cells contain at least two and often several nucleoli, fat droplets are rare. A mixture of BW1-J and Fao cells were treated with inactive Sendai Virus to produce hybrids at 1 hybrid colony/50 BW1-J cells.

배양 배지

Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS)

계대배양 정규 작업

Split semi-confluent cultures (70%) 1:50 i.e. seeding at 2x1000 - 5x1000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Media change three times a week.

기타 정보

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