M. dunni (Clone III8C)

94101211

• UNSPSC 코드: 41106514

속성

• 생물학적 소스: mouse tail
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Fibroblast-like
• 제품: Not specified
• 수용체: Ecotropic Murine leukemia virus (MuLV)
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Mouse tail, normal fibroblast

세포주 설명

Derived from the tail tissue of a normal female mouse, Mus dunni. The cells were cloned at passage 14 using Tererasiki plates. M. dunni (Clone III8C) supports the replication of all four classes of murine leukaemia viruses (MuLV); ecotropic, amphotropic, xenotropic and mink cell focus-forming viruses (MCF), showing cytopathic effect. The cells lack most endogenous murine leukaemia virus-related sequences. They are resistant to infection by Moloney MuLV, due to post-translational modifications of the ecotropic MuLV receptor. Confluent cultures will remain more firmly attached in RPMI 1640 or DMEM + 10% FBS during virus assays.

애플리케이션

Detection of replication-competent retroviruses in gene therapy.

배양 배지

McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 1-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

기타 정보

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