SK-N-F1

94092304, human nerve (bone marrow metastasis), Epithelial-like

• UNSPSC 코드: 41106514
• 생물학적 소스: human nerve (bone marrow metastasis)
• 성장 모드: Adherent/aggregates in suspension
• 핵형: Aneuploid
• 형태학: Epithelial-like
• 제품: Not specified
• 수용체: Transferrin receptor
• 관련 질환(들): metastasis

속성

• 제품명: SK-N-F1, 94092304
• 생물학적 소스: human nerve (bone marrow metastasis)
• 성장 모드: Adherent/aggregates in suspension
• 핵형: Aneuploid
• 형태학: Epithelial-like
• 제품: Not specified
• 수용체: Transferrin receptor
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): metastasis
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human neuroblastoma

세포주 설명

SK-N-F1 has been obtained from a bone marrow metastasis of a male patient with neuroblastoma. The poorly differentiated and slow growing cells secrete different neuroblastoma specific peptides and synthesise choline acetyltransferase. Reactivity of anti-leukaemia-associated and anti-foetal brain-associated monoclonal antibodies could be shown. Epithelial-like and aggregate-forming populations.

애플리케이션

Carcinogenesis studies, cytotoxicity assays.

DNA 프로파일

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11,14
D16S539: 11,12
D5S818: 12,13
D7S820: 8,9
THO1: 6,9.3
TPOX: 8,9
vWA: 16,17

배양 배지

DMEM + 4mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 2-4x10,000 cells/ml using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Clumped cells have tendency to float.

기타 정보

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