Colo 794

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 94072237, human skin, Not specified

• UNSPSC 코드: 41106514

속성

• 생물학적 소스: human skin
• 포장: tube of 5 μg 94072237-DNA-5UG; pkg of vial of cells 94072237-1VL
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Not specified
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): metastasis
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human melanoma

세포주 설명

Colo 794 was established from a 14 year old male patient with a malignant melanoma. The melanoma showed rapid growth and metastases in the patient and the biopsy was taken before therapy from a subcutaneous metastasis. The cell line was eradicated from mycoplasma at ECACC.COLO 794 and COLO 800 (Sigma Catalogue number 93051123) were both derived from the same 14 year old male This has been confirmed by STR profiling.

애플리케이션

Cancer studies

DNA 프로파일

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10
D13S317: 11,13
D16S539: 11
D5S818: 11
D7S820: 10,11
THO1: 6
TPOX: 11
vWA: 16,20

배양 배지

RPMI 1640+ 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:2 to 1:3 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C; cells can show extensive blebs

기타 정보

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