KYSE-410

94072023, human esophagus, Epitheloid

• 동의어(들): KYSE0410 Cells, KYSE410 Cells
• UNSPSC 코드: 41106514

속성

• 제품명: KYSE-410, 94072023
• 생물학적 소스: human esophagus
• 성장 모드: Adherent
• 핵형: Hypotetraploid with 4% polyploidy
• 형태학: Epitheloid
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human oesophageal squamous cell carcinoma

세포주 설명

Established from the poorly differentiated invasive oesophageal squamous cell carcinoma resected from the cervical oesophagus of a 51-year old Japanese man prior to treatment. Reported to overexpress heparin binding growth factor (hst-1) and cyclin D. This cell line has been mycoplasma eradicated at ECACC. By short tandem repeat (STR)-PCR analysis the Y chromosome could not be detected in this cell line. It is a known phenomenon that SSC cell lines can lose their Y chromosome.

DNA 프로파일

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 11
D16S539: 10,12
D5S818: 13
D7S820: 12
THO1: 8
TPOX: 8,11
vWA: 16,18

배양 배지

RPMI 1640 + 2mM Glutamine + 5-10% Foetal Bovine Serum (FBS)

계대배양 정규 작업

Split confluent cultures 1:5 to 1:10 twice a week i.e. seeding at 2-4x10,000 cells/cm² using trypsin/EDTA;5% CO₂; 37°C. Doubling time ~45h

기타 정보

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