NCI-H727

94060303, human lung, Epithelial

• UNSPSC 코드: 41106514

속성

• product name: NCI-H727, 94060303
• 생물학적 소스: human lung
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Epithelial
• 제품: Synthesise the peptide neuromedin B (NMB) (0.1 pmol/mg protein).
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human lung non-small cell carcinoma

세포주 설명

A non-small cell carcinoma of the lung from a 65 year old Caucasian female taken, prior to treatment. The cells express easily detectable levels of p53 mRNA compared to levels found in normal lung. The cells are not able to synthesise gastrin releasing peptide (GRP). NCI-H727 is the best differentiated available bronchial carcinoid cell line. Any proposed commercial use of these cells or products must first be negotiated with the University of Texas. More details available upon request.

애플리케이션

Synthesis of NMB.

DNA 프로파일

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 11
D16S539: 11,13
D5S818: 11,12
D7S820: 8,10
THO1: 8
TPOX: 8
vWA: 14,15

배양 배지

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

기타 정보

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