TGP 49

94022427

• UNSPSC 코드: 41106514

속성

• 생물학적 소스: mouse pancreas
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Trabecular
• 제품: Somatostatin (low levels)
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Mouse pancreatic acinar carcinoma

세포주 설명

TGP 49 has been isolated from an acinar cell carcinoma arising in the pancreas of a transgenic Tg(E1a-1-SV40E)Bri18 mouse. These mice carry a pseudogene construct composed of a pancreatic specific elastase-1 promoter linked to the SV40T antigen and thereby represent an animal model for pancreatic carcinogenesis. TGP 49 secretes low amounts of somatostatin, it is one of a series of cell lines isolated from pancreatic tumours deposited at ECACC. They have been reported not to secrete amylase or lipase but do secrete trypsin. Differentiation characteristics fluctuate from time to time in culture. Trabecular and clumps of small cells.

애플리케이션

Study of endocrine pancreas carcinomas, hormone assays.

배양 배지

DMEM:Ham′s F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

기타 정보

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