COLO 800

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 93051123, human skin, Fibroblast

• UNSPSC 코드: 41106514

속성

• 생물학적 소스: human skin
• 포장: tube of 5 μg 93051123-DNA-5UG; pkg of vial of cells 93051123-1VL
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Fibroblast
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human melanoma

세포주 설명

Derived from a subcutaneous nodule of a tumour from a 14-year-old male. Cells display remarkable blebbing.COLO 800 and COLO 794 (Sigma Catalogue number 94072237) were both derived from the same 14 year old male This has been confirmed by STR profiling.

애플리케이션

Tumourigenicity studies

DNA 프로파일

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10
D13S317: 11,13
D16S539: 11
D5S818: 11
D7S820: 10,11
THO1: 6
TPOX: 11
vWA: 16,20

배양 배지

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 35°C; 5% CO₂.

기타 정보

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