JEG3

92120308, human placenta, Epithelial

• UNSPSC 코드: 41106514

속성

• product name: JEG3, 92120308
• 생물학적 소스: human placenta
• 성장 모드: Adherent
• 핵형: modal no. 71
• 형태학: Epithelial
• 제품: Human chorionic gonadotrophin (hCG), somatomammotrophin
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human choriocarcinoma

세포주 설명

One of 6 clones derived from cells implanted into a hamster cheek pouch and then propagated on irradiated feeder layers of human fibroblasts. Cell line releases chorionic gonadotrophin and somatomammotrophin and progesterone. It is also able to transform steroid precursors to oestrone and oestradiol. JEG3 has the same DNA profile as BeWo (Sigma Catalogue number 86082803); it was established by serial cloning of BeWo .

배양 배지

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin, 5% CO₂; 37°C. Vacuolisation will occur at confluency.

기타 정보

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.