ND7/23 Cell Line

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 92090903, Mouse x Rat Hybridoma nerve, Neuronal

• 동의어(들): ND7/23 Cells
• UNSPSC 코드: 41106514

속성

• 생물학적 소스: Mouse x Rat Hybridoma nerve
• 포장: tube of 5 μg 92090903-DNA-5UG; pkg of vial of cells 92090903-1VL
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Neuronal
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Mouse neuroblastoma x Rat neurone hybrid

세포주 설명

Mouse neuroblastoma (N18 tg 2) x rat dorsal root ganglion neurone hybrid cell line produced by PEG mediated cell fusion.

애플리케이션

Mouse neuroblastoma (N18 tg 2) and rat dorsal root glanglia produced by PEG-mediated fusion.

Recommended culture medium: DMEM + 2 mM L-Glutamine + 10% FBS

Subculture: Split at 70-80% confluency, approx. 1:3 to 1:6, seed at 1-3x10⁴ viable cells/cm² at 37°C and 5% CO₂. Dissociate cells by aspiration or gentle tapping. Cells are lightly adherent, no trypsinization is necessary.

배양 배지

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² by either aspirating or gently tapping the cells loose; 5% CO₂; 37°C. Cells are lightly attached therefore use of trypsin is unnecessary.

기타 정보

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