HT29 gluc C1

92012401, human colon, Epithelial

• UNSPSC 코드: 41106514

속성

• product name: HT29 gluc C1, 92012401
• 생물학적 소스: human colon
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Epithelial
• 제품: Elevated levels of cystic fibrosis transmembrane conductance regulator (CFTR) protein
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human Caucasian colon adenocarcinoma

세포주 설명

A subclone of the parent HT29-18 differentiated to specific cell types for studies on cystic fibrosis.

배양 배지

DMEM + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 44mM Sodium Bicarbonate (NaHCO₃) + 10mg/l Transferrin + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:2 to 1:8, i.e. seeding at 1-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

기타 정보

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