CFPAC-1

91112501, human pancreas (liver metastasis), Epithelial

• UNSPSC 코드: 41106514

속성

• product name: CFPAC-1, 91112501
• 생물학적 소스: human pancreas (liver metastasis)
• 성장 모드: Adherent
• 핵형: Hyperdiploid, (65-75 chromosomes)
• 형태학: Epithelial
• 제품: Not specified
• 수용체: Cystic fibrosis trans-membrane regulator (CFTR)
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): metastasis
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human Caucasian pancreatic adenocarcinoma

세포주 설명

CFPAC-1 is a ductal pancreatic adenocarcinoma derived by differential trypsinisation of explant cultures from a metastatic lesion in the liver of a 26 year old caucasian male with cystic fibrosis (CF). The cell line expresses cystic fibrosis trans-membrane regulator (CFTR) and ion transport properties consistent with the CF defect. CFPAC-1 cells show an epithelial morphology and polarisation with apical microvilli. Expression of cytokeratin and oncofoetal antigens characteristic of pancreatic duct cells have been described. The cells express the CF gene and show the most common CF mutation, deletion of three nucleotides result in a phenylalanine-508 deletion. CFPAC-1 cells are also tumorigenic in nude mice.

애플리케이션

Cystic fibrosis studies, genetic analysis

배양 배지

IDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x 10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

기타 정보

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