TRA-171

90120514, insect larvae, Fibroblast-like

• UNSPSC 코드: 41106514

속성

• 제품명: TRA-171, 90120514
• 생물학적 소스: insect larvae
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Fibroblast-like
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Insect larvae, mosquito

세포주 설명

The cell line TRA-171 has been established from freshly hatched larvae of Toxorhynchites amboinensis. After eggs had been surface-sterilised, they were allowed to hatch in MM / VP12 medium and were mechanically damaged at the anterior or posterior ends of the body in 0.25% trypsin. After washing and cultivation at 22 - 25°C small patches of cells were visible leading to the isolation of the cell line TRA-171 after 72 days in culture. The cells have been adapted to grow in a 1:1 mixture of Leibovitz L-15 and Mitsuhashi and Maramorosch media plus supplements. Four serotypes of laboratory adapted and three type strains (1, 2 and 3) of un-adapted dengue virus will replicate in the cells at 28°C.

애플리케이션

Virus studies: Dengue virus

배양 배지

L15: Mitsuhashi and Maramorosch Insect Medium (1:1) + 2mM Glutamine + 0.05% BSA + 1% NEAA + 10% Heat Inactivated Foetal Bovine Serum (HI-FBS)

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4 x 10,000 cells/cm² using 0.25% trypsin for 5 minutes only at 28°C, after quenching the trypsin with fresh medium dislodge cells by gentle scraping;  CO₂ not required; 28°C. Fr

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