GS-109-V-20

90110505, human skin, Fibroblast-like

• UNSPSC 코드: 41106514

속성

• product name: GS-109-V-20, 90110505
• 생물학적 소스: human skin
• 성장 모드: Adherent
• 핵형: 2n = 46
• 형태학: Fibroblast-like
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human Caucasian skin fibroblast Gardner′s syndrome

세포주 설명

Derived from skin from the upper arm of an 18 year old Caucasian male with Gardner′s syndrome is an autosomal dominant condition which predisposes to carcinoma and multiple polyps of the colon. Cells exist in an initiated state rather than a normal or transformed state. Normal diploid karyotype.

애플리케이션

Tumourigenicity studies

배양 배지

EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split semi-confluent cultures 1:2 i.e. seeding at 4x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. Monolayer will form in a 25cm² flask in 1 week.

기타 정보

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