CHP 4 (W.W.)

90102537, human skin, Fibroblast

• UNSPSC 코드: 41106514

속성

• product name: CHP 4 (W.W.), 90102537
• 생물학적 소스: human skin
• 성장 모드: Adherent
• 핵형: 2n = 46
• 형태학: Fibroblast
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human skin asymptomatic Galactosemia

세포주 설명

Derived from skin tissue taken from an 8 year old male with asymptomatic galactosemia, despite lacking red cell galactose-1-phosphate uridyl transferase. Sibling of CHP 3 (M.W.) (Sigma catalogue no. 90102536) with the same enzyme deficiency that was symptomatic. Cultures will retain characteristics for at least 30 passages. Ethnicity: Black.

애플리케이션

Enzyme deficiency

배양 배지

DMEM + 2mM Glutamine + 2% Non Essential Amino Acids (NEAA) + 2% Vitamins + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-4x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

기타 정보

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