Detroit 510

90102531, human skin, Fibroblast

• UNSPSC 코드: 41106514

속성

• 제품명: Detroit 510, 90102531
• 생물학적 소스: human skin
• 성장 모드: Adherent
• 핵형: 2n = 46
• 형태학: Fibroblast
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human Caucasian skin Galactosemia

세포주 설명

Derived from skin tissue of a 9 month old Caucasian female with Galactosemia. Galactose-1-phosphate transferase negative.

애플리케이션

Virus studies

DNA 프로파일

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 11,12
D16S539: 11,12
D5S818: 10,12
D7S820: 8,12
THO1: 7,9.3
TPOX: 8
vWA: 15,17

배양 배지

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 0.1% Lactalbumin hydrolysate (LH) + 10%Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. Finite lifespan, up to 30 population doublings are expected.

기타 정보

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