BCL1 Clone CW13.20-3B3

90061904, mouse blood, Lymphoblast

• UNSPSC 코드: 41106514

속성

• product name: BCL1 Clone CW13.20-3B3, 90061904
• 생물학적 소스: mouse blood
• 성장 모드: Suspension
• 핵형: Not specified
• 형태학: Lymphoblast
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Mouse BALB/c B cell leukaemia

세포주 설명

Derived from BCL1 by limiting dilution and does not spontaneously secrete IgM when cultured in pre-tested batches of FBS. The line is useful for studying soluble factors involved in B-cell differentiation.

애플리케이션

B-cell differentiation studies

배양 배지

RPMI 1640 + 2mM Glutamine + 1% Sodium Pyruvate (NaP) + 1% Non Essential Amino Acids (NEAA) + 0.05mM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Cells attach lightly to surface which should be tapped sharply to dislodge the cells. Do not allow cells to become confluent, subculture at 75% to 90% confluency; free-floating aggregates acc

기타 정보

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