1BR.3.GN

90020509, human skin, Fibroblast

• UNSPSC 코드: 41106514

속성

• product name: 1BR.3.GN, 90020509
• 생물학적 소스: human skin
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Fibroblast
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human skin fibroblast, transformed

세포주 설명

Derived from a normal fibroblast (1BR3 Sigma Catalogue number. 90011801) transformed with the plasmids pSV3gpt and pSV3neo. The cells were selected for pSV2neo only.The genetic identity of 1BR3 (90011801), 1BR.3.G (90020507), 1BR.3.GN (90020508), and 1BR.3.N (90020509) has been confirmed by STR profiling at ECACC.

애플리케이션

DNA repair and cell growth studies

배양 배지

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

기타 정보

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