1BR.3.N

90020508, human skin, Fibroblast

• UNSPSC 코드: 41106514
• 생물학적 소스: human skin
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Fibroblast
• 제품: Not specified
• 수용체: Not specified

속성

• 제품명: 1BR.3.N, 90020508
• 생물학적 소스: human skin
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Fibroblast
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human skin fibroblast, transformed

세포주 설명

Derived from a normal fibroblast (1BR3 Sigma Catalogue number. 90011801) transformed with the plasmid pSV2neo. The genetic identity of 1BR3 (90011801), 1BR.3.G (90020507), 1BR.3.GN (90020508), and 1BR.3.N (90020509) has been confirmed by STR profiling at ECACC.

애플리케이션

DNA repair and cell growth studies

DNA 프로파일

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 9,13
D13S317: 9,12
D16S539: 12,14
D5S818: 12
D7S820: 8,10
THO1: 9.3
TPOX: 11
vWA: 14,15

배양 배지

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

기타 정보

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.