175BR

90011863, human skin, Fibroblast

• UNSPSC 코드: 41106514

속성

• product name: 175BR, 90011863
• 생물학적 소스: human skin
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Fibroblast
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human skin fibroblast

세포주 설명

Established from an adult male with primary carcinoma. The donor was the brother of the donor of 174BR (Sigma Catalogue number. 90011895).

애플리케이션

DNA repair studies

배양 배지

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

기타 정보

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