LNCap Clone FGC Cell Line human

89110211, from human prostate(carcinoma)

• 동의어(들): LNCaP-FGC Cells
• UNSPSC 코드: 41106514

속성

• 생물학적 소스: human prostate (carcinoma)
• 성장 모드: Adherent
• 핵형: Pseudodiploid male; seven marker chromosomes, modal number 46, range 33 to 91
• 형태학: Epithelial-like
• 제품: Prostate acid phosphatase, prostate specific antigen
• 수용체: Androgen, estrogen
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): metastasis
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human Caucasian prostate carcinoma

세포주 설명

Derived from a metastasis at the left supraclavicular lymph node of a 50 year old patient with a confirmed diagnosis of metastatic prostate carcinoma. Growth and acid phosphatase production is affected by 5-alpha-dihydrotestosterone. They do not form a uniform monolayer and attach only lightly to the substrate. When shipped, cells detach from flask and can either be incubated 24-48 hours to allow attachment or be collected by centrifugation (150xg, 15 minutes) and reseeded.

애플리케이션

LNCap Clone FGC Cell Line has been used:

• to study the effect of sex steroid hormones in regulating the expression of oligoadenylate synthetase 1 (OAS1)
• to study the role of curcumin and resveratrol in redox control and ability to promote apoptosis
• to investigate low molecular weight thiols present in the deproteinized extract of the cell line after derivatization

배양 배지

RPMI 1640 + 2mM Glutamine + 1.0 mM sodium pyruvate + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.i.e. seeding at 1-3 x 10,000 cells/cm² Cells grow slowly in clusters and can be disaggregated by repeated pipetting. After subculture cells may take up to 48 hours to reattach. Do not disturb during this period. Medium is rapidly acidified, therefore change twice per week.

기타 정보

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