추천 제품
product name
Sf9 Cell Line from Spodoptera frugiperda pupal ovarian tissue, 89070101
생물학적 소스
insect ovary
성장 모드
Adherent
핵형
Not specified
형태학
Spherical
제품
Not specified
수용체
Not specified
기술
cell culture | mammalian: suitable
배송 상태
dry ice
저장 온도
−196°C
세포주 기원
Spodoptera frugiperda pupal ovarian tissue
세포주 설명
Derived from pupal ovarian tissue of spodoptera frugiperda. The cells are highly susceptible to Baculovirus infection and are used in the production of protein products genetically manipulated into Baculovirus vector systems.
애플리케이션
Sf9 cell line has been used for Baculovirus viral studies. It has also been used for in vitro kynurenine-3-monooxygenase (KMO) inhibition assay.
배양 배지
TC100 + 2mM Glutamine. (The medium can be supplemented with 10% Foetal Bovine Serum (FBS) if required).
계대배양 정규 작업
Maintain cultures between 3x100,000 to 1x1,000,000 cells/ml; 27°C. Gently resuspend cells in old culture medium by dislodging the cells by pipetting, or gentle agitation, and resuspend in fresh medium. Cells should stick to the culture flask. Freeze in 9% DMSO/91% FBS. Cultures may take up to 10 days to recover normal growth characteristics following cryopreservation and resuscitation.
기타 정보
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시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
문서
Eukaryotic cell lines aid in complex post-translational modifications and folding of recombinant proteins. Find suitable recombinant protein production cell lines at sigmaaldrich.com
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