R2C

89031606, rat testis, Epithelial

• UNSPSC 코드: 41106514

속성

• product name: R2C, 89031606
• 생물학적 소스: rat testis
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Epithelial
• 제품: Steroid hormones; 20-hydroxypregn-4-en-3-one; progesterone
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Rat testis Leydig cell tumour

세포주 설명

A rat testis Leydig cell tumour cell line derived from a 2-month-old male WFu rat. Virus susceptibility: herpes simplex; vaccinia; pseudorabies; vesicular stomatitis (Indiana). Resistant to poliovirus 2. Stimulation by other hormones or cAMP is not required for hormone secretion.

배양 배지

HAMS F10 + 2mM Glutamine + 15% Horse serum + 2.5% Foetal Bovine Serum (FBS). Do not use heat-inactivated serum.

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin/EDTA;5% CO₂; 37°C.

기타 정보

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