I-10

89031605, mouse testis, Epithelial

• UNSPSC 코드: 41106514

속성

• product name: I-10, 89031605
• 생물학적 소스: mouse testis
• 성장 모드: Adherent
• 핵형: 2n = 40, hyperdiploid
• 형태학: Epithelial
• 제품: Steroids
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Mouse BALB/c Leydig cell testicular tumour

세포주 설명

Derived from a Leydig cell testicular tumour of a BALB/c mouse. The cells secrete steroids; this increases at saturation density. Secretion should be retained for over 50 passages. Exposure to 0.00001M cAMP stimulates secretion 4-5 fold. The major products are progesterone and its derivative 20 α-hydroxy-4-pregnen-3-one. It does not respond to interstitial cell stimulating hormone (ICSH) but is stimulated by cyclic adenosine monophosphate (AMP).

애플리케이션

Tumourigenicity studies

배양 배지

Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 6x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

기타 정보

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