A375 Cell Line human

88113005, human skin, Epithelial

• 동의어(들): A-375 Cells, A375-MEL Cells
• UNSPSC 코드: 41106514

속성

• product name: A375 Cell Line human, 88113005
• 생물학적 소스: human skin
• 설명: Human malignant melanoma
• 성장 모드: Adherent
• 핵형: Modal no. = 62 (hypotriploid) 9 marker chromosomes. Normal N2, N6 and N22 present.
• 형태학: Epithelial
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human malignant melanoma

세포주 설명

Derived from a 54 year old female with malignant melanoma. The cell line produces rapidly growing amelanotic melanomas in anti-thymocyte serum treated NIH Swiss mice.

애플리케이션

A375 cell line has been used to obtain paracrine factors for prolonged culture of mesenchymal stromal cells (MSCs). It has also been used to study oncolytic activity of the peptide LTX-315.

Tumourigenicity studies

배양 배지

DMEM + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

기타 정보

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