추천 제품
제품명
NCTC clone 929, 88102702
생물학적 소스
mouse connective tissue
성장 모드
Adherent
핵형
2n = 40
형태학
Fibroblast
제품
Not specified
수용체
Not specified
기술
cell culture | mammalian: suitable
배송 상태
dry ice
저장 온도
−196°C
세포주 기원
Mouse C3H/An connective tissue
세포주 설명
From parental strain L which was derived from normal subcutaneous areolar and adipose tissue of 100 day old male C3H/An. Used in development of replicate culture techniques, chemically defined media, fluid suspension culture methods and virus assays. Susceptible to pseudorabies virus and vesicular stomatitis (Indiana strain) virus.
애플리케이션
Replication, biochemical, neoplastic transformation, nutritional, metabolic enzyme, morphological, chromosome, immunologic, radiation, toxicologic and virus studies
배양 배지
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
계대배양 정규 작업
Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Freeze in 10% glycerol/90% FBS. After resuscitation cells may take up to 4 days to establish growth.
기타 정보
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