AV3 (HeLa derivative)

88102402, human cervix, Epithelial

• UNSPSC 코드: 41106514

속성

• 제품명: AV3 (HeLa derivative), 88102402
• 생물학적 소스: human cervix
• 포장: tube of 5 μg 88102402-DNA-5UG; pkg of vial of cells 88102402-1VL
• 성장 모드: Adherent
• 핵형: 2n = 46
• 형태학: Epithelial
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human cervix carcinoma

세포주 설명

This cell line was originally derived from normal human amnion. The cells are mainly used for virus studies (susceptible to poliovirus 1). HeLa marker chromosomes and type A G6PD have been detected. This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line must be considered to be derived from HeLa.

애플리케이션

Virus studies: poliovirus 1

DNA 프로파일

STR-PCR Data: Amelogenin: X
CSF1PO: 9,10
D13S317: 14
D16S539: 9,10
D5S818: 11,12
D7S820: 12
THO1: 7
TPOX: 8,12
vWA: 16,18

배양 배지

EMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5x100,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

기타 정보

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