DEM human cell line

88052007, IHW Number: 9007

• UNSPSC 코드: 41106514

속성

• 생물학적 소스: human blood
• 민족성: German
• 성별: male
• 성장 모드: Suspension
• 핵형: Not specified
• 형태학: Lymphoblastoid
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice

설명

일반 설명

Consanguineous: No

Homozygous: No

세포주 설명

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

DNA 프로파일

A: 201
Cw: 602
B: 5701
HLA DRA: 101
HLA DRB1: 0401 1602
HLA DRB4: 101
HLA DRB5: 2
HLA DRB6: 202
HLA DQA1: 0102 03
HLA DQB1: 0502 0302
HLA DPA1: 1
HLA DPB1: 0301 0401

혈청학적 프로파일

HLA A: 2
HLA A ief: 2.2
HLA C: 6
HLA B: 57
HLA B ief: 57.2
Bw4/bw6: 4
HLA DR: 4 16
HLA DR51: 51
HLA DR53: 53
HLA DQ: 5 8
HLA DP: 3 4

배양 배지

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

계대배양 정규 작업

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

기타 정보

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면책조항

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.