CRI-G5

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC 코드: 41106514

속성

• 생물학적 소스: rat pancreas
• 포장: tube of 5 μg 88031603-DNA-5UG; pkg of vial of cells 88031603-1VL
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Epithelial
• 제품: Insulin and glucagon
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Rat NEDH islet tumour

세포주 설명

Established from a NEDH rat transplantable islet cell tumour. The cells secrete insulin and glucagon. This cell line can be used in place of CRI-D11 (Sigma Catalogue number. 88031604).

애플리케이션

Hormone studies

배양 배지

DMEM + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. The cells tend to grow in clumps and ribbons.

기타 정보

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