KG-1

86111306, human bone marrow, Myeloblast-like

• 동의어(들): KG1 Cells
• UNSPSC 코드: 41106514

속성

• 제품명: KG-1, 86111306
• 생물학적 소스: human bone marrow
• 성장 모드: Suspension
• 핵형: 2n = 46, diploid
• 형태학: Myeloblast-like
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human Caucasian bone marrow myelogenous leukaemia

세포주 설명

Derived from the bone marrow aspirate of a 59 year old male Caucasian with erythroleukemia that became acute myelogenous leukaemia. The cells form colonies in soft-agar in the presence of colony stimulating factor (CSF). KG-1 cells resemble acute myelogenous leukaemia showing considerable pleomorphism with a predominance of myeloblasts and promyelocytes. A small percentage of the cells are mature granulocytes with the occasional presence of macrophages and eosinophils. They do not express surface Ig or EBV antigens and lack specific markers for lymphocytic cells.

애플리케이션

KG-1 has been used to extract total RNA and use them as templates to determine the 5′ end of promyelocytic leukemia zinc finger (PLZF) transcripts.

DNA 프로파일

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 7
D13S317: 11,12
D16S539: 10,11
D5S818: 13
D7S820: 8,10
THO1: 7,8
TPOX: 7,9
vWA: 14,19

배양 배지

IDMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

기타 정보

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