CRFK (BVD Ag negative)

86093002, feline kidney, Epithelial

• UNSPSC 코드: 41106514

속성

• product name: CRFK (BVD Ag negative), 86093002
• 생물학적 소스: feline kidney
• 성장 모드: Adherent
• 핵형: Hypodiploid, modal no 36
• 형태학: Epithelial
• 제품: Carcinoembryonic antigen (CEA)
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Cat kidney

세포주 설명

CRFK cells were isolated from the cortical portion of the kidneys of a 10-12 week old normal female domestic cat. CRFK are extensively used in feline virus research. Susceptible to herpesvirus and feline picornaviruses producing CPE. BVD Ag negative.

애플리케이션

Crandell feline kidney cells (CRFK) (BVD Ag negative) have been used in viral studies.

배양 배지

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

기타 정보

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