BC3H1

86093001, mouse brain, Fibroblast

• UNSPSC 코드: 41106514

속성

• 제품명: BC3H1, 86093001
• 생물학적 소스: mouse brain
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Fibroblast
• 제품: Acetylcholine
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Mouse brain tumour

세포주 설명

Established in 1974 from a mouse brain neoplasm tumour induced by nitrosoethylurea, possess many properties characteristic of smooth muscle. Since then it has been suggested that BC3H1 is a skeletal line of ectodermal origin that is defective for commitment to terminal differentiation. The two distinct morphologies typical of this cell line are large spheroid cells and smooth-muscle-like cells. The fusiform is the more common.

애플리케이션

Acetylcholine receptor studies.

배양 배지

DMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Spilt confluent cultures 1:3 to 1:10 i.e. seeding at 1-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

기타 정보

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