STO

86032003, mouse embryo, Fibroblast

• UNSPSC 코드: 41106514

속성

• product name: STO, 86032003
• 생물학적 소스: mouse embryo
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Fibroblast
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Mouse SIM embryo fibroblast

세포주 설명

Selected for 6-thioguanine and ouabain resistance; sensitive to HAT; HPRT negative. Used routinely as feeder layers prepared by irradiation or mitomycinc treatment for maintenance of teratocarcinoma stem cells in the undifferentiated state.

애플리케이션

Oubain resistance, feeder layer

배양 배지

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

기타 정보

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