P3HR-1

86010701, human lymph, Lymphoblast

• UNSPSC 코드: 41106514

속성

• product name: P3HR-1, 86010701
• 생물학적 소스: human lymph
• 성장 모드: Suspension
• 핵형: 2n = 46, hyperdiploid
• 형태학: Lymphoblast
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human Burkitt′s Lymphoma

세포주 설명

Clonally-derived sub-line of JIYOYE (Sigma Catalogue number. 88071302) containing high levels of EBV. Has been successfully hybridised with the human lymphoid cell line K562 to form the hybrid PUTKO-1. ACDP class II pathogen and should be handled under laboratory containment level 2. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

DNA 프로파일

STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 12
D16S539: 10,11
D5S818: 12
D7S820: 8,10
THO1: 7,9
TPOX: 6,8
vWA: 15,19

배양 배지

RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

기타 정보

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