A673

85111504, human muscle, Epithelial-like

• UNSPSC 코드: 41106514

속성

• product name: A673, 85111504
• 생물학적 소스: human muscle
• 성장 모드: Adherent
• 핵형: Near Diploid
• 형태학: Epithelial-like
• 제품: Transforming growth factor (Tramnsforming Growth Factor (TGF)) - ß
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human Ewing sarcoma

세포주 설명

Derived from a 15 year old female patient. The cells form colonies on soft agar and tumours in antithymocyte serum treated mice.

The cell line A673 was originally described in 1973 as derived from a rhabdomyosarcoma, however, more recent analysis of the cells using advanced diagnostic techniques determined the cells to have characteristics of Ewing sarcoma rather than rhabdomyosarcoma. See citations for more information about this tumour status.

애플리케이션

Tumourigenicity studies

DNA 프로파일

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 8,13
D16S539: 11
D5S818: 11,12
D7S820: 10,12
THO1: 9.3
TPOX: 8
vWA: 15,18

배양 배지

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:10, i.e., seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.

기타 정보

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