BRL 3A

85111503, rat liver, Epithelial

• UNSPSC 코드: 41106514
• 생물학적 소스: rat liver
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Epithelial
• 제품: MSA factors
• 수용체: Not specified

속성

• 제품명: BRL 3A, 85111503
• 생물학적 소스: rat liver
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Epithelial
• 제품: MSA factors
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Buffalo Rat liver

세포주 설명

Originally isolated by primary cloning. Serum-free medium conditioned by the cells produces a family of polypeptides termed multiplication stimulating activity (MSA).

애플리케이션

Growth factor studies

배양 배지

Coon′s modified Ham′s F12 + 2mM Glutamine + 5% Foetal Bovine Serum (FBS) or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol + 5% Foetal Bovine Serum (FBS) + 2mM Glutamine.

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

기타 정보

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