Do-11-10

85082301, mouse unknown/unspecified, Not specified

• UNSPSC 코드: 41106514

속성

• product name: Do-11-10, from mouse unknown/unspecified, 85082301
• 생물학적 소스: mouse unknown/unspecified
• 성장 모드: Suspension
• 핵형: Not specified
• 형태학: Not specified
• 제품: Not specified
• 수용체: Not specified
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Mouse BALB/c (H-2d) T cells x Mouse AKR Thyoma, BW5147

세포주 설명

Do-11-11 was produced by fusion of the antigen-specific I region-restricted (H-2d) BALB/c T-cell blasts with the AKR thyoma BW5147. This murine helper T cell hybridoma is specific for chicken ovalbumin (cOva) restricted to I-Ad and cross reacts with cOva I-antibody. The hybrid can be stimulated to secrete Interleukin 2 (IL-2) or to act as an antigen-specific helper T-cell. Expression of IL-2 can be lost by long-term culture of growth at high densities.

배양 배지

DMEM or RPMI 1640 + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS).

계대배양 정규 작업

Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO₂; 37 °C. Cells should not be grown at high density; avoid long-term culture as this might lead to change of cell receptors.

기타 정보

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