WI 38VA13 Subline 2RA

85062512, human lung (fetal), Epithelial

• UNSPSC 코드: 41106514

속성

• product name: WI 38VA13 Subline 2RA, 85062512
• 생물학적 소스: human lung (fetal)
• 성장 모드: Adherent
• 핵형: 2n = 46, hyperdiploid, modal no. 73-78
• 형태학: Epithelial
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human Caucasian foetal lung, SV40 transformed

세포주 설명

An SV40 transformed derivative of WI 38. The cells are characterised by loss of contact inhibition, unlimited proliferation and the presence of SV40 antigens.

애플리케이션

Transformation and virus studies: SV40

배양 배지

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

기타 정보

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