H4-II-E-C3

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85061112, rat liver, Epithelial

• UNSPSC 코드: 41106514

속성

• 생물학적 소스: rat liver
• 포장: tube of 5 μg 85061112-DNA-5UG; pkg of vial of cells 85061112-1VL
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Epithelial
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Rat liver hepatoma

세포주 설명

From H-35 Reuber hepatoma. Tyrosine aminotransferase positive. Synthesises several serum proteins including albumin, transferrin, prothrombin. The presence of type C virus has been shown and can readily be verified by electron microscopy.

애플리케이션

Enzymatic and virus studies: Type C virus

배양 배지

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

기타 정보

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