HeLa B

85060701, human cervix, Epithelial

• UNSPSC 코드: 41106514
• 생물학적 소스: human cervix
• 성장 모드: Adherent
• 핵형: Aneuploid
• 형태학: Epithelial
• 제품: Not specified
• 수용체: Not specified
• 관련 질환(들): cancer

속성

• 제품명: HeLa B, 85060701
• 생물학적 소스: human cervix
• 성장 모드: Adherent
• 핵형: Aneuploid
• 형태학: Epithelial
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human cervix carcinoma

세포주 설명

A derivative of HeLa, the first aneuploid, epithelial-like line derived from human tissue and maintained by serial subculture. Identified as a contaminant in many subsequently derived lines, due to poor culture techniques. Susceptible to poliovirus 1 and Ad 3.

애플리케이션

Tumourigenicity and virus studies: poliovirus 1 and Ad 3

배양 배지

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

기타 정보

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