MRC-5 SV1 TG1

85042501, human lung (fetal), Fibroblast

• UNSPSC 코드: 41106514

속성

• product name: MRC-5 SV1 TG1, 85042501
• 생물학적 소스: human lung (fetal)
• 성장 모드: Adherent
• 핵형: Modal no. = 60-80 hyperdiploid
• 형태학: Fibroblast
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human foetal lung, SV40 transformed

세포주 설명

A clonal derivative of an SV40 transformed permanent line of MRC-5. Selection was made using X-ray induction in the presence of 2μg/ml thioguanine. The cells are HAT sensitive.

배양 배지

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

기타 정보

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