DAUDI Cell Line human

85011437, human blood, Lymphoblast

• 동의어(들): DAUDI Cell Culture, Human DAUDI Cells
• UNSPSC 코드: 41106514

속성

• product name: DAUDI Cell Line human, 85011437, African-American Burkitt′s lymphoma, from human blood
• 생물학적 소스: human blood
• 성장 모드: Suspension
• 핵형: 2n = 46, diploid
• 형태학: Lymphoblast
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human Burkitt′s lymphoma

세포주 설명

Derived from a 16 year old male. Positive for EBNA, carries EBV markers, complement receptors, surface bound immunoglobulin and surface markers for the Fc fragment of IgG. The cells should be handled under laboratory containment level 2. Ethnicity: Black.

애플리케이션

Tumourigenicity and Virus studies: EBV used to study leukemogenesis mechanisms.

DNA 프로파일

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 12
D13S317: 11,12
D16S539: 10,12
D5S818: 8,13
D7S820: 8,10
THO1: 6,7
TPOX: 8,11
vWA: 15,17

배양 배지

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Maintain cultures between 2-9x100,000 cells/ml; 5% CO₂; 37°C. Will increase 12 fold within 10-14 days provided fresh medium is added at 48-72 hour intervals. Cell growth is slow on recovery from frozen. Resuscitate using 20% FBS and keep culture flask standing up until exponential growth is established.

기타 정보

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